Evaluation of chromosome organization and microtubule arrangement in goat (capra aegragrus) oocytes after vitrification, in vitro maturation and fertilization, and early embryo development

نویسندگان

چکیده

Objective: Evaluate the use of Ethylene Glycol (EG), Dimethyl Sulfoxide (DMSO), Sucrose and Fetal Bovine Serum (FBS) as cryoprotectants their effect on organization chromosomes arrangement microtubules, during vitrification process in goat oocytes matured vitro development preimplantation embryos produced vitro.
 Design/methodology/approach: In were divided into 3 groups (control group, cryoprotectant exposed vitrified group). A mixture 15% EG, DMSO, 0.4 M sucrose 20% FBS was used for using Cryotop device. warmed afterwards each group two more groups. Both subjected to immunofluorescence, first observe damage microtubules second embryo development.
 Results: The combined did not prevent cryoinjuries embryos, since disrupted before blastocyst stage by stopping cleavage at morula stage. This disruption associated with chromosome decondensation absence a microtubule network, thereby hindering chromosomal segregation.
 Limitations study/implications: conventional production.
 Findings/conclusions: caprine improve vitro.

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ژورنال

عنوان ژورنال: Agro productividad

سال: 2022

ISSN: ['2594-0252']

DOI: https://doi.org/10.32854/agrop.v15i11.2420